clem

Adaptive 3D cryogenic correlative light imaging of native biology using laser free confocal system

Cryogenic light microscopy is an important part a correlative imaging workflow, allowing confirmation of localised molecules of interest prior to processing for downstream analysis such ultrastructural or compositional assessment or in short: It is a key mapping step.

Here, the authors describe adaptation of a standard upright widefield microscope into a cryogenic 3D laser-free confocal system using the Linkam CMS196. We demonstrate the necessary sample preparation steps followed by confocal imaging of biological cells and tissues.

Access the full version here: https://analyticalscience.wiley.com/do/10.1002/was.00170086

Nizamudeen, Zubair Ahmed, et al. "Adaptive 3D cryogenic correlative light imaging of native biology using laser free confocal system. Wiley Analytical Science

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Imaging of Vitrified Biological Specimens by Confocal Cryo Fluorescence Microscopy and Cryo FIB /SEM Tomography

Researchers use the Linkam CMS196 as part of a Confocal Cryo Fluorescence Microscopy setup to image vitrified biological samples.

Access the PDF version here.

Schertel, Andreas, et al. "Imaging of Vitrified Biological Specimens by Confocal Cryo Fluorescence Microscopy and Cryo FIB/SEM Tomography." European Microscopy Congress 2016: Proceedings. Weinheim, Germany: Wiley‐VCH Verlag GmbH & Co. KGaA, 2016.

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